International Livestock Research Institute (ILRI)
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About the position
The position focuses on construct development and performing genomic DNA extractions, PCR, Southern blotting, qRT-PCR, sequencing, mutation detection, and maintaining bacterial cultures. The successful candidate will work closely with a multidisciplinary team of scientists and contribute to ongoing research in plant genome editing and trait enhancement.
Key Responsibilities
Develop genome editing and RNAi constructs.
Perform genomic DNA extraction from various transgenic and gene edited events of date palm, sugarcane or any other crops for molecular analysis.
Conduct PCR (Polymerase Chain Reaction) and qRT-PCR (quantitative Real-Time PCR) to assess gene expression and mutation verification.
Perform mutation detection assays, including T7 endonuclease and band-shift analysis.
Carry out Southern blot analysis for gene copy number determination and transgene integration.
Sequence analysis for detection and validation of gene edits using techniques such as Sanger or next-generation sequencing.
Maintain and propagate bacterial cultures for plasmid DNA preparations.
Contribute to the maintenance of gene-edited plant lines in the lab and greenhouse.
Ensure accurate data collection, analysis, and record-keeping for experimental results.
Assist in preparing reports, presentations, and manuscripts related to the research findings.
Collaborate with other team members and provide technical support in molecular techniques.
Perform any other duty assigned
Requirements
Bachelor's degree or diploma in molecular biology, Biotechnology, Genetics, or a related field.
Relevant professional certification.
Two years relevant experience with degree or four years' relevant experience with diploma. Experience with in silico gene cloning tools such as Benchling and SnapGene
Hands-on experience with molecular techniques such as DNA extraction, PCR, qRT-PCR, and sequencing.
Familiarity with Southern blotting and mutation detection assays is a plus.
Ability to maintain detailed lab records and follow protocols with precision.
Strong organizational and time management skills.
Excellent interpersonal and communication skills for working in a collaborative research environment.